About CRISPR Streamline

CRISPR/Cas9 is a tool which is undergoing rapid growth in the fields of genetic engineering due to its ability to locate and mutate programmable genomic sequences in vivo. However, suboptimal gRNA design can lead to frequent off-target splicing events at unintended genome locations which can greatly decrease CRISPR/Cas9 efficiency.Numerous bioinformatics programs exist which attempt to predict potential off-target sites within genomes, however, no such programs exist to further predict potential phenotypic effects from those off-target splices despite the value such information holds to scientists.

Crispr Streamline is a command-line program which builds on the previously established off-target prediction tool FlashFry by passing output genome locations to the ClinVar database as a means of predicting consequential phenotypic effects. This program accumulates in a web-based tool which is freely accessible for those performing CRISPR/Cas9 experiments or gRNA validation.

We currently have a web application scientists and researchers can use to produce off-target phenotype information on chosen genome sequences.