We require users to upload FASTA files containing their sequence of interest.
Once a job is submitted, users will see a job ID appear in the URL of the submission page, save this ID and once your results have been generated, return to: https://birg.dev/streamline/job/<your job ID>. Here you will have three options to download your results, either as a .txt, .csv, or .xlsx file.
Depending on the size of the FASTA file submitted, as well as the number of offtargets found in the submitted file, CRISPR Streamline can take anywhere from a few minutes to half an hour to complete.
All phenotypic information is queried from ClinVar through the UCSC Genome Browser.
Feel free to email the team and request a specific genome be included in the tool. We are always iterating and improving so your feedback and suggestions are welcome.